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Core Developer Notes

What is a Cytosol Landscape?

ClpXP Control Module: Deployment in Nucleus Cytosol

Development of a Control Module in Nucleus Cytosol Reactions

Characterizing the Limited Operational Lifetime of Cytosol Reactions

SecYEG-Based Membrane Translation Module in Synthetic Cells

Development of a Membrane Translation Module via Reconstitution of the SecYEG Translocon for Enhanced Membrane Protein Integration in Liposomes

Using platemaps to analyze and share data

Intro to Kinetics Analysis of Plate Reader Experiments

Nucleus OnePot PURE

Nucleus OnePot PURE Protocol and Results

ClpXP Control Module: Deployment in PURE Cells

ClpXP Control Module: Deployment in PURE Cells

Nucleus Base Cell Testing

Evaluation of Nucleus Base Cell Through deGFP Expression

ClpXP Control Module: Deployment in PURE

ClpXP Control Module: Deployment in PURE

DNA toolkit - The T7 promoter collection

The first entry to a DNA resource specific to PURE

First Nucleus Cytosol Testing

Evaluation of Nucleus Cytosol Through deGFP Expression

Cx43 Cell: DNA Validation

Cx43 Cell: DNA Validation

Integrating PPK Module in PURE Cells

PPK Module increases protein expression in PURE Cell

Nucleus OnePot PURE Replication

Work in progress to replicate and improve upon the Nucleus OnePot PURE protocol

The Developer Cell Control Module: Protein Degradation by ClpXP

The Developer Cell Control Module: Protein Degradation by ClpXP

PPK Module testing in PURE

PPK Module increases protein expression

Developer Cell: Project Introduction

Developer Cell: Project Introduction